Abstract

APPLICATIONS OF HTPLC IN ANALYSIS OF PHYTOPHARMACEUTICALS: REVIEW

V. R. Biradar*, R. D. Chakole and M. S. Charde

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Abstract

Most of the plants provide various pharmacological activity including categories tannins, resins, essential oil, alkaloids, anthraquinones, cardiac glycoside. These all are categorized phytochemicals detected by various chromatographic method like HPTLC, GC, HPLC. The review included various applications HPTLC technique for phytopharmaceuticals and quality control of herbal botanical formulations; It also included various precise and accurate HPTLC parameters like mobile phase, flow rate, RF values, PH conditions, stationary phase for estimation of various phytopharmaceuticals. HPTLC is sophisticated, automated, and improved form of TLC. The checklist of botanical plants along with their specific validation is still biased. The tradition method is less scientific and poor. The HPTLC method was optimized to resolve the major compound from other components. This technique most popular due to multiple sample handling, visual chromatogram, and best separation technique. The phytopharmaceuticals are plant derived compound having various pharmacological action. HPTLC is helpful to identify and quantify the active constituents with minimizing cross contamination and time. It is also advanced in reproducible analysis, visual evaluation which are help to researcher to analysis of phytopharmaceuticals.

Keywords: Phytopharmaceuticals, HPTLC, Herbals.