Abstract

APPLICATIVE BIODEGRADATION STUDY OF EGG ALBUMIN NANOSPHERES BY ALKALINE PROTEASE FOR RELEASE OF ENCAPSULATED CICER ARIETINUM AMYLASE IN WASHING AS BIO-ACTIVE DETERGENT ADDITIVE

Kirti Rani*

Abstract

In present work, Cicer arietinum amylase was encapsulated through covalent coupling by glutaraldehyde into chemically modified egg albumin nanospheres using toluene as stabilizer. Biodegradation of chemically modified egg albumin nanospheres was carried out by using varying concentration of alkaline protease (5U, 10U, 15U, 20U, 25U, 30U, 35U, 40U) for its controlled and sustained release of encapsulated enzyme. Storage stability and thermal stability of encapsulated enzyme was increased up to 6 months and up to 70ºC respectively as compared to free enzyme (24 hours only and 50ºC respectively) which enhanced industrial application of the bio-active enzyme loaded egg albumin nanopreparation. Further, chemically modified egg albumin nanospheres of encapsulated Cicer arietinum amylase with alkaline protease were used with detergents for washing of stained cloths having tough dried strains of ball point pen, white board marker and permanent marker and not to be easily vanished with detergents in one wash. Hence, the bio-active mixture of Cicer arietinum amylase loaded chemically modified egg albumin nanospheres with alkaline protease were used with detergents powder (Aerial, Surf excel, Wheel and Tide) for washing of these dry tough strains (ball point pen, white board marker and permanent marker) lead to washing off strains very fast with clear results in one wash without soaking as compared to results of washing of stained cloths with detergents only.

Keywords: Cicer arietinum amylase, egg albumin, Glutaraldehyde, Encapsulated, Emulsified.