Abstract

STUDIES ON PHYTOCHEMICAL, ANTIOXIDANT, PROXIMATE AND ELEMENTAL ANALYSIS OF HYBANTHUS ENNEASPERMUS (L) F. MUELL LEAF.

K. Thyaga Raju*, B. Namratha Rani, Kamala. K and G. Sujatha

Abstract

This study has investigated the phytochemical constituent, antioxidant properties, proximate and elemental analysis of different solvent leaf extracts (Hexane, ethyl acetate, ethanol, water) of H.enneaspermus known for its therapeutic value in folklore medicines. The Phytochemical and qualitative analysis on different extracts of H.enneaspermus have showed the presence of alkaloids, flavonoids, carbohydrates, steroids, tannins, glycosides and terpenoids. Ethanolic extract has demonstrated significant radical scavenging, antioxidant activity and highest phenolic content compared to hexane, ethyl acetate, and aqueous extracts. The dried leaf powder proximate analysis has revealed the presence of 90.6% of dry matter, 9.31% of moisture content, 35.1% of crude fiber, 12.5% of total ash, 0.3% of acid insoluble ash and 6.8% crude fat. Elemental analysis was done by ICP-OES and it revealed the presence of calcium (4.7mg), magnesium (4.2mg), zinc (3.0mg), phosphorus (3.2mg) and potassium (11.4mg) per gram of dry leaf powder.

Keywords: Hybanthus enneaspermus, DPPH, Proximate analysis, ICP-OES.