Abstract

GC-MS ANALYSIS AND "IN VITRO" ANTICANCER ASSAY OF METHANOLIC LEAF EXTRACT OF ASYSTASIA GANGETICA (L.). T.ANDERSON AGAINST A549 HUMAN LUNG CANCER CELL LINE

Tamilselvan V* and Rajeswari M

Abstract

As lung cancer is one of the most notorious cancer worldwide and the mortality rate is expected to increase drastically in developing countries like China and India, the present investigation has been made in order to get a remedy from medicinal plant. In the present study the "in vitro" anticancer assay of Methanolic Leaf extract of Asystasia gangetica (MLAG) was made against A549 human lung cancer cell lines. The phytoconstituents of the plant has also been identified with the help of GC-MS (Gas Chromatography – Mass Spectrometry) analysis. Numerous anticancer compounds like epithilones, Psi-Psi carotenes, baicalines, veratramines, vitamin-D acetate, colchicines, isocorydines have been identified from the MLAG. The IC50 value of MLAG was found to be 122.85μg/ml. It was also found that the cytotoxic value was concentration dependent. As the concentration of MLAG increased the cytotoxic value has found to be increased. The anti-proliferative nature of the plant might be due to the combination of multiple anticancer compounds.

Keywords: Asystasia gangetica, GC-MS, MTT Assay, A549 Human Lung Cell Line.