Abstract

STUDIES ON ANTIMICROBIAL ACTIVITY BY EXTRACELLULAR AND INTRACELLULAR YEAST PROTEINS

Dheyaa Shakir Mashrea, Ganesh Dama, Anand M, Prasad M.P,
Balasubramanian Sathyamurthy*

Abstract

In the present study yeasts have been isolated from different sources like juice samples and soil. The antimicrobial activity for each of the isolate was checked against B.subtilis, Pseudomonas, E.coli, Klebseilla, Salmonella, Serratia, Staphyloccous and Streptococcus. The Isolates showed antimicrobial activity against most of the pathogens and had a greater zone of inhibition. Klebseilla, P.aeuriginosa and Salmonella was inhibited by all the isolates proving that Saccharomyces cerevisiae can be used as of the antimicrobial agent against those microorganisms. The proteins were purified from the isolates which showed better inhibition activity and they were checked whether the proteins were produced extracellular or intracellular. It was found that both intracellular and extracellular proteins had a greater impact on the inhibition of the microorganisms and the phosphate buffer A and ammonium sulphate precipitation method for the purification of the protein had an improved inhibition when compared to the other methods used to purify the proteins. The SDS-PAGE was carried out for the protein from the isolates and the intracellular proteins and extracellular proteins showed a wide range of proteins with different molecular weights.

Keywords: S.cerevasiea, Antimicrobial activity, Proteins, SDS-PAGE.