Abstract

SILK DYE EFFLUENT INDUCED CHANGE IN ULTRASTRUCTURE OF LIVER ON SWISS ALBINO MICE MUS MUSCULUS AND APPLICATION OF MORINGA OLEIFERA LEAF POWDER

Dr. Serina Khatun*

.

Abstract

The silk dye (Azo dye) waste is one of the most potential harmful chemicals liberated in the environment in an unexpected manner. Silk dye waste is widely used as a potent dyeing of yarn and fabrics in many countries and has been shown to produce some adverse health effects. This work focuses primarily on the effects of Moringa oleifera leaf extract on liver of silk dye effluent induced surface ultrastructure in Swiss albino mice Mus musculus. The liver tissue has been taken an account for surface ultrastructural study. The mice were divided into 5 Groups i.e. Group I (Control), Group II (fed with 50% silk dye), Group III (fed with 100% silk dye), Group IV (mice fed with 50% dye treated with M. oleifera leaves powder), Group V (mice fed with 100% dye treated with M. oleifera leaves powder) have been taken for experiment. The dose of silk dye was 2ml/day to both groups II and III and M. oleifera leaf is given as per the standard dose (300mg/kg b.w) to both animals of group IV and V. Administration of silk dye waste result were demonstrated atrophy of liver, enlargement of the sinusoidal space, vacuole formations in hepatocytes, infiltrations with haemorrhage in hepatic tissue but used of Moringa oleifera leafs powder it was significantly recovered the damage tissues has been observed. This study suggested that the extract may have beneficial effect on surface ultrastructural constituents such as Liver.

Keywords: Silk dye waste, Moringa oleifera leaf powder, Liver, Mus musculus, SEM for Ultra-Structure, Toxicity assessment.