Abstract

PREPARATION AND EVALUATION OF BEDAQUILINE LOADED MICROSPHERES BY SOLVENT EVAPORATION TECHNIQUE

J. Adlin Jino Nesalin, Manu Kumar M. S.* and Sachith M. P.

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Abstract

The Bedaquiline loaded Ethyl cellulose microspheres were prepared by solvent evaporation technique. Microspheres of different core: coat ratio were formulated and evaluated for loading efficiency, particle size, zeta potential, solubility studies, in vitro drug release, kinetic studies and stability studies. The prepared microspheres have a particle diameter ranging approximately from 285-578 μm and a zeta potential of the ideal formulation FS3 is found to be 61.2 mV. There was a steady increase in the entrapment efficiency on increasing the polymer concentration in the formulations. The invitro drug release profile of the formulation was in the range 76.23 to 91.11 % for 120 min (fig 6). the batch FS3 showed the fastest dissolution rate, with approximately 91% of the drug being released within 120min.No appreciable difference was observed in the drug content of product which were stored at 5˚±3°C and room temperature (30º± 2ºC/65%RH), and 40± 2ºC/75%RH, (after 3 months storage (as per QA1(R)) of stability studies. According to the data obtained, this ethyl cellulose- based delivery system opens new and interesting perspectives as drug carriers.

Keywords: Solvent evaporation method, Microspheres, Bedaquiline, Ethylcellulose, solubility, Tuberculosis.