Abstract

EVALUATION OF NEPHRO-PROTECTIVE EFFECT OF DPP4 INHIBITOR AND ANTIOXIDANT AGAINST GENTAMYCIN INDUCED NEPHROTOXICITY IN ALBINO RATS

Shamal J. Kadam*, Vitthal J. Chaware and Vivekkumar K. Redasani

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Abstract

Background:- Nephrotoxicity is a global health challenge of vast proportion around the world. Recent studies demonstrated the renoprotective effects of two dipeptidyl peptidase-4 (DPP-4) inhibitors, saxagliptin and linagliptin, against gentamycin-induced renal injury. However, none of these studies investigated the combination of DPP 4 inhibitor and antioxidant. This prompted us to test this hypothesis and to assess, for the first time, the potential reno-protective effect of DPP- 4 inhibitor and antioxidant. Objective:- This study aimed to investigate the potential protective effect of DPP-4 inhibitor and antioxidant on gentamycin-induced nephrotoxicity. Method:- Nephrotoxicity was induced in the rats with Gentamycin (100mg/kg). All animals except normal control were intraperitoneally administered with gentamycin at a dose of 100mg/kg once daily for 10 days. Respective treatment were started from day 2nd till day 14th (2 weeks). On the 15th day, blood samples were collected through retro-orbital plexus under anesthesia. Serum was separated to measure creatinine, BUN, uric acid, proteins & MDA (Malondialdehyde). Body weight were also recorded. Result:- Administration of combination of DDP-4 inhibitor and antioxidant ameliorated gentamycin induced renal injury and restored renal functional, oxidative, inflammatory, apoptotic & histopathological changes. Coclusion:- These findings suggest that combination of DDP-4 inhibitor and antioxidant treatment attenuate renal dysfunction and structural damage through the reduction of oxidative stress, mitochondrial dysfuction and apoptosis in the kidney.

Keywords: Nephrotoxicity, Gentamycin, DDP-4 inhibitor, antioxidant, Renal Biomarkers.