Abstract

POTENTIATION OF EFFECTS OF PROPRANOLOL AND HEPARIN BY ANTIOXIDANT IN ADRENALINE INDUCED MYOCARDIAL INFARCTION IN RATS

Manasvi P. Patil*, Vitthal J. Chaware and Vivekkumar K. Redasani

.

Abstract

Background: Myocardial Infarction also known as heart attack is still a major cause of morbidity and mortality around the world. Vitamin C is a powerful antioxidant that can strengthen body's natural defenses and propranolol and heparin are the drugs which affect the cardiovascular system. So there is need to see if Propranolol, heparin, vitamin C and combinations of vitamin C with low doses of propranolol and heparin can show cardioprotection against adrenaline induced myocardial infraction (MI) in rats. Objective: To study cardioprotective effect of Vitamin C and its combination with low dose of propranolol and heparin against adrenaline induced myocardial infarction (MI) in rats. Materials and Methods: Rats were divided randomly into seven groups (6 rats each); Group I-Normal control; Group II-Toxicant control (adrenaline treated group); Group III-rats were pre-treated with propranolol (10 mg/kg p.o) for 10 days (from day 12 to day 21); Group IV-rats were pre-treated with heparin (500 units/kg) for 2 days (on 20th and 21th day); Group V-rats were pretreated with vitamin C (40 mg/kg i.p) for 21 days; Group VI-rats were pretreated with vitamin C ( 40 mg/kg ) and propranolol (5 mg/kg) for 21 days and from 12 to 21 days respectively; Group VII-rats were pretreated with vitamin C (40 mg/kg) and heparin (250 units/kg) for 21 days and on 20th -21th day respectively. The rat model of myocardial infarction was produced by injecting adrenaline (2 mg/kg b.w.) subcutaneously into all animals (except the control) twice at a 24-hour interval on days 20th and 21th day of the experiment. The cardiac markers tests were done at the conclusion of the trial. Results: Treatment with Adrenaline showed significantly increased in biochemical parameters (CK-MB, LDH, SGOT, Troponin-I). Prior treatment with propranolol, heparin, vitamin C and its combination with low doses of propranolol and heparin showed significant alteration in these parameters. Conclusion: The results of present study highlights that propranolol, heparin and vitamin C when given alone showed cardioprotection but combination of vitamin C with Propranolol and Heparin with low doses has showed better cardioprotection based on the alterations of cardiac biomarkers.

Keywords: Myocardial infarction, Adrenaline, Oxidative stress, Inflammation, Propranolol, Heparin, Vitamin C, Cardiac biomarkers.