Abstract

ANTAGONISTIC ACTIVITY OF PROBIOTIC AND SEA WEED EXTRACT AGAINST VEGETATIVE GROWTH FOR SOME FUNGI AND ZEARALENONE PRODUCTION

Dr. Shatha Ali Shafiq

Abstract

The current study was carried out to evaluate the use of Iraqi probiotic and sea weed extract Ascophyum nodosum (foreign product) against some phytopathogenic fungi and reduce the production of Zearalenone which it is produced by Fusarium graminearium in liquid medium Yeast Extract Sucrose (YES). Using different concentrations from probiotic (0.0, 0.5, 1, 1.5, 2)% w/ v and sea weed extract (0.0, 2, 4, 6, 8) % v/v .It has all the concentrations of probiotic showed high efficiency in inhibiting the growth of fungi, especially at concentrations (1, 1.5) inhibition percentages were 100% against Fusarium graminearium and Pythium aphanidermatum. While the results of sea weed extract was shown differences in inhibition percentage for all concentration based on the type of fungus except Penicillum digitatum and Pythium aphanidermatum the inhibition percentages were 100% at higher concentrations (1, 1.5,2). The research presented here mainly concerns the inhibition of fungal growth Fusarium graminearum and Zearalenone (ZEN) production in liquid medium using higher concentrations of probiotic (1.5, 2) % w/v and sea weed extract (4,8) % v/v. The dry weight of Fusarium graminearum was evaluated (gm) and the results of probiotic treatment was more efficiency in inhibition of fungal growth and inhibition the level of Zearalenone production completely 100 % compared with sea weed extract inhibition percentages of fungal growth were (0.33, 0.47) % and (74.2, 79.4) % the reduction percentage of Zearalenone at ( 4, 8 ) % v/v respectively.

Keywords: Fusarium graminearium, Pythium aphanidermatum, Zearalenone.