Abstract

COMPARATIVE TOXICITY ASSESSMENT OF ORGANOCHLORINE AND DIMETHOATE ON SERUM PROTEIN AND AMINO ACID PROFILE OF SWISS ALBINO MICE MUS MUSCULUS AND RELATED HEPATIC ANOMALIES: IN VIVO STUDY

Prakriti Verma* and G. B. Chand

Abstract

Swiss Albino mice Mus musculus was exposed to endosulfan@2 mg/kg. body wt., rogor @20 mg / Kg. body wt. separately & a combination of endosulfan(@1 mg /kg. body wt) and rogor (@10 mg/ Kg .body wt.) for 7, 4 & 21 days respectively. At the termination of each exposure, blood sample were collected & serum was extracted to analyze the quantitative estimation of total protein and amino acid composition by standard method of Moore & Stein using thin layer chromatography. The hepatic tissues were processed for light microscopic studies. The results showed an increasing trend of serum protein content at all the exposure level of rogor while a decreasing trend was reported against endosulfan exposure. An abrupt trend of fluctuation in serum protein was reported in combined exposure. Amino acid profile of control group showed a very high percentage of valine, serine, aspartic acid, tyrosine & glutamic acid while a very diminished concentration of these amino acids were found in the serum of treated groups. The hepatic tissues showed corresponding anomalies in different treated groups. Hence abrupt fluctuation in serum protein, diminished amino acid contents can be considered as sensitive indices of toxicity generated by agrochemicals in mice.

Keywords: Mus musculus, Endosulfan, Rogor, Hepatic tissues, Light microscopy, TLC, Total protein, Amino acid.