Abstract

INSULIN NANOPARTICLES LOADED SUPPOSITORES INTENDED FOR THE SYSTEMIC DRUG DELIVERY

Ch. Meghana, A. V. Jithan and *H. Parameshwar

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Abstract

The main objective of the present work was to develop colon targeted insulin nanoparticle loaded suppositories intended for systemic delivery of the active. This delivery provides sustained release delivery of the drugs through the rectal route. Nanoparticles based on PLGA were loaded with insulin using w/o/w solvent evaporation technique. The prepared nanoparticles were characterized for various in vitro properties. Particle size and charge were measured using zeta sizer and SEM. Integrity of the drug at the end of formulation development was determined using FTIR, DSC and XRPD. The duration of drug release was determined using in vitro release testing methods. After selecting suitable nanoparticle formulation, they were incorporated into cocoa butter as suppositories. The drug release was also determined at the end of formulation development and a suitable formulation was proposed. The results demonstrated that sustained released of insulin was observed over one week with improved stability of insulin. These suppository formulations loaded with insulin nanoparticles are intended for colon delivery so as to achieve systemic levels of insulin as an alternate route of delivery of this drug.

Keywords: Colon drug delivery, insulin, lyophilization, suppository.