Abstract

QUERCETIN ALLEVIATES THE SIDE EFFECTS OF CLOMID IN THIOACETAMIDE INDUCED INFERTILITY IN RATS

Walaa Awad, Shadia M. Kadry and Marwa T. Hassen*

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Abstract

The current work was accomplished to assess the effect of clomid or/ and quercetin on thioacetamide induced infertility in rats. Seventy adult male rats were separated into 2 main groups A as control group & group B as infertility group. GroupA (30 rats) are divided into 3 groups: Group 1A: Control (TAA solvent), Group2A: Rats treated with clomid., Group 3A: Rats treated with quercetin. Infertility group B is induced in male rats (40 rats) by i.p. injection of TAA. These animals are injected by TAA (300 mg/kg) 2 times with 24 hour intervals. The considered infertility rats are divided into 4 groups: Group 1B: Maintained untreated, Group 2B: Rats treated with clomid (0.35mg/kg Clomid i.p.), Group 3B: Rats treated with quercetin (the normal group with Que content of 50 mg/kg bw daily by oral gavage), Group 4B: Rats were treated with both clomid accompanied with querectin. The results confirmed that TAA decreased hormones (FSH, LH, Testesterone), glutathione and catalase while, malondialdehyde was increased. The histopathological study showed several damages in testes tissue. The immunohistochemical invagination revealed an increment in caspase 3 and TNF-α expression. clomid or/ and quercetin administration induced improvement of the biochemical studies. The testes tissue was enhanced in histological, and immunohistochemical investigations.

Keywords: Infertility; clomid; querectin; testes.