Abstract

PHYTOCHEMICAL SCREENING AND THE ANTIMICROBIAL ACTIVITY OF THE LEAVES OF AZADIRACHTA INDICA, A. JUSS

Rahul Mathur*, Rohit Kumar, Subhash Yadav, Rohit Kumar Yadav, Roobi Patel, Sameer Khan, Sandeep Yadav, Dr. Jagdish Rathi and Shweta Gogate

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Abstract

The leaves of Azadirachta indica, A. Juss were collected. The leaves were cleaned. One portion of the leaves were shade dried for one week and pulverized to coarse powder. For the preparation of the ethanolic, methanolic and aqueous extract, 1g of the dried ground plant material was weighed and extract was prepared by refluxing with 100 ml of the solvent. Qualitative analysis of phytochemicals was done for carbohydrates, phenols, tannins, flavonoids, saponins, glycosides, cardiac glycosides and alkaloids. The Phytochemical screening we found that carbohydrate, tannins, alkaloids, glycosides and flavonoids were present. Antibacterial activities of Methanol, Ethanol and Aqueous extracts of leaves (300μg/ml) were evaluated in the term of zone of inhibition. It was observed that extracts were effective against Staphylococcus Aureus and Pseudomonas Aeruginosa, while Cefotaxime was used as standard. Antifungal activities of Methanol, Ethanol and Aqueous extracts of leaves (300μg/ml) were evaluated in the term of zone of inhibition. It was observed that extracts effective against both candida albicans and aspergilus niger while. Overall, anti bacterial activity was possessed by extract of flowers and seeds but anti fungal activity was possessed by extract of flowers only.

Keywords: Phytochemical, screening, antimicrobial, azadirachta indica.