Abstract

EVALUATION OF WOUND HEALING POTENCY OF ‘CASSIA AURICULATA LINN’ FLOWER EXTRACT USING IN-VITRO METHOD

Dr. Shambhulingaiah H. M.*, Mounika S., Akshtha MJM., Anusha K., Neenu Raj, Ranjith Kumar P. B.

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Abstract

Wounds are ‗The Silent Epidemic‘ Wounds have a variety of causes; some arise from surgical intervention, some are the result of injury, and others are a consequence of extrinsic factors, such as pressure or shear, or underlying conditions such as diabetes or vascular disease. Wound healing mechanism is obligatory to regain the lost tissue and maintain tissue homeostasis. New tissue formation is a complex process, which involves multiple steps such as inflammation, angiogenesis, granulation tissue formation, re-epithelialisation, and ECM reconstruction. Fibroblasts are most abundant cells in skin tissue and the major functions of these cells during wound healing include, rupturing of fibrin clots, generation of extracellular matrix (ECM) components and collagen structures that support the tissue homeostasis. In vitro cell based scratch assay is an appropriate and inexpensive method for initial understanding of wound healing potential of medicinal plant extracts. The current study was aimed at investigating the wound healing capacity of hydroalcoholic flower extracts of Cassia auriculata (L.) by using scratch assay as a primary model, where proliferative and migratory capabilities of test compounds could be monitored through microscopy studies. A scratch assay involves growing a cell monolayer to confluence in a multiwall assay plate; creating a ‗wound‘—a cell-free zone in the monolayer— into which cells can migrate; and monitoring the recolonization of the scratched region to quantify cell migration, In our study, we observed that L929 cells migrated better towards the artificially created wound when treated with the Cassia auriculata flower extract. This suggests that the extract accelerates wound healing by inducing the migration of fibroblasts, may be due to the presence of various phytoconstituents present in it.

Keywords: Wound healing, Fibroblast cells, Scartch assay, Senna auriculata.