Abstract

GEL CHROMATOGRAPHY: A REVIEW

*Kunal B. Patil, Sayali A. Deshmukh, Mayur M. More, Dhananjay Chaudhari, Neha Jaiswal and Shardha Amrutkar

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Abstract

Gel filtration chromatography is a popular and versatile technique that enables the efficient separation of proteins and biological molecules in high yields. Here, the basics of the method are explained and conventional matrix types are contrasted. Selection of suitable operating conditions and application of the Method is also discussed

Keywords: Gel-fi ltration chromatography, Gel-permeation, Gelexclusion, Size-exclusion, Molecular-sieve, Operating conditions, Separations.