Abstract

FORMULATION OF ZINGIBERENE LOADED MICROPARTICLES FOR EXTENDED RELEASE

*Ravina Vashisth, Deepak Basedia and Balkrishna Dubey

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Abstract

The objective of the present research work was to encapsulate zingiberene in polymeric microparticles in order to improve the half life of zingiberene. Zingiberene was isolated from ginger oil using column chromatographic technique using hexane-diethyl ether (97:3,v/v) as the eluting solvent. The absorption maximum of zingiberene in UV spectroscopy was found to be 232 nm It was found that zingiberene was miscible with organic solvents whereas immiscible with water. Stirring time, stirring speed and the concentration of surfactant were pivotal for obtaining the particles of optimum particle size and spherical shape and the polymer concentration of 10 %w/v was able to produce denser particles. The yield of the zingiberene loaded dried microspheres was found to be 74% and the average particle size of the drug loaded microparticles was found to be 37 ± 1.8 μm. The percentage of drug loading in the microparticles was found to be 6% with entrapment efficiency of 66%. The in vitro drug release suggested a sustained release of zingiberene from the microparticles with around 63 % drug released in the medium over a period of 10h.

Keywords: Zingiberene, microspheres, release, column chromatography, sodium dodecyl sulfate.