Abstract

EVALUATION OF DELONIX REGIA (LEAVES) WAS USED TO INVESTIGATE FOR THEIR PROTECTIVE ABILITY OF THE KIDNEY AGAINST GENTAMYCIN-INDUCED NEPHROTOXICITY

Satendra Dhakad*, Dr. Ravi Prakash Yadav, Dr. Brajesh Ku. Arjariya, Rahim Khan, Mohd. Jalaluddin and Laxminarayan Dhakad

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Abstract

The present study aims to examine the potential beneficial effects of Delonix regia (leaves) against gentamicin-induced renal injury in vitro and in vivo, as well as to investigate the main underlying pathogenic mechanisms. Gentamycin is a widely used aminoglycoside antibiotic that has proven efficiency against life-threatening Gram-negative bacterial infections. Collected material was washed thoroughly in running tap water, rinsed in distilled water and shade dried in open air and ground to powder. The air-dried and powdered defatted marc of the drugs was subjected to extraction with hydroalcoholic solvent (ethanol: water: 80: 20). Tested for the presence of alkaloids, glycosides, flavonoids, and carbohydrates etc. In conclusion Preliminary phtochemical investigation on 80:20 hydroalcoholic extract of Delonix regia leaves showed the presence of bioactive compounds such as Glycosides, flavonoids, diterpenes, phenolics, carbohydrates, saponins compounds and alkaloids, proteins and amino acids are absent. A group of natural polyphenols called “flavanoids” are of most popular interest because reserchers have found them to be of health contributing potenial. The total phenol content was 1.690 (mg GAE/100mg of leaves extract while the flavonoid content was 1.305 (mg QE/100mg) of hydroalacolic extract. Therefore, Delonix regia leaves represented a novel therapeutic tool in the prevention and treatment of gentamicin-induced nephrotoxicity in the clinical setting.

Keywords: Gentamycin, nephrotoxicity, Delonix regia, kidney.