Abstract

COMPARATIVE POTENTIALS OF HUNTERIA UMBELLATA FRUIT EXTRACT IN PANCREATIC AND LIVER FUNCTION IN ALOXAN AND PHENYLHYDRAZINE INDUCED DIABETES AND ANEMIA IN MALE WISTAR RATS

Dr. Solomon M. Uvoh*, Dr. Emily Kiridi G. E., Dr. Blessing Dum-Awara L., Okuroemi Henrietta O.

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Abstract

Biochemical analysis was conducted to determine the effect of Hunteria Umbellata whole fruit extract on pancreatic and liver condition of male wistar rats. Seventy wistar rats were divided into seven groups of ten animals each fed with animal feeds and water ad libitum. The group1 serve as the normal control, group2 was administered 200mg/kg fruit extract without induction, group3 and 4 was treated at 400 and 200mg/kg fruit extract while group5 was treated with insulin after aloxan induction. Group 6 and 7 induced with phenylhydrazine was treated at 500 and 250mg/kg fruit extract of Hunteria Umbellata. The biochemical indices of liver and pancreatic determine include AST, ALT, ALP, TP, ALB, CB, TB and insulin. Result from this study shows decreased insulin level but mostly in group2 to group4. Aspatate aminotransferase (AST) normal values was regain in group3 to group5 treated at 400-200mg/kg aqueous fruit extract and insulin after aloxan monohydrate induction. However, group2 treated at 200mg/kg fruit extract without aloxan induction has the lowest AST concentration level of 30.50U/L compared with the normal control group (39.00U/L) and other groups. The alanine aminotransferase (ALT) concentration was maintain within normal range among the groups treated with the fruit extract though in gruop7 induced with phenylhydrazine appears to have lower value of 20.50U/L compared with other groups. Furthermore, total protein, albumin, total bilirubin and conjugated bilirubin level were all improved after receiving the fruit extract treatment though the alkaline phosphatase concentration decreased in group 2,4,6 and group7 treated at 200 ,400 and 200mg/kg body weight extract daily compared with group3 and group5 treated at 400mg/kg fruit extract and insulin daily. This study has shown that liver function can be improved in aloxan monohydrate and phenylhydrazine induced diabetes and anemic male wistar rats treated with Hunteria Umbellata aqueous whole fruit extract daily despite decrease in insulin level.

Keywords: Insulin, liver, phenylhydrazine, aloxan, conjugated bilirubin, total protein.