Abstract

METHODOLOGY FOR IN-VITRO EVALUATION OF ANTIOXIDANT PROPERTIES- A REVIEW

Abhang Dhananjay Chandrakant*, Shirsath Shantnu Hemant, Walke Priya Arun and Gholap Suraj Machindra

Abstract

This article provides details concerning various methods that are useful for carrying out the In-vitro antioxidant activity. After going through a large number of research articles and abstracts published, thus far, for calculating the antioxidant action of several samples of scientific interest and came across the number of in-vitro methods used to perform an antioxidant activity, from which 20 methods were described in this review article. It provides basic information about Antioxidants and their classification under Enzymatic and Nonenzymatic antioxidants classes. It emphasizes the method simplicity, the time necessary, reagent requirement and instrumentation, methodology, that makes to determine and which method to be followed to carry out for checking antioxidant properties depend on the feasibility and afforded to determine it. It also provides a comparison of the most frequently used methods like DPPH a radical scavenging method, among all those methods which act as a good reference for further research. This article will be an encyclopedic prepared reference for them that are excited about the study of Antioxidants.

Keywords: Antioxidants, in-vitro methods, scavenging activity, DPPH, Reducing power.