Abstract

UV SPECTROSCOPY METHOD DEVELOPMENT AND VALIDATION FOR SIMULTANEOUS ESTIMATION OF CARVEDILOL AND LISINOPRIL IN SYNTHETIC MIXTURE

Nena Jaiswal*, Mitali Dalwadi, Priyanka Malani and Umesh Upadhyay

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Abstract

Sensitive, precise, accurate and simple UV spectrophotometric methods have been developed for Analytical Method for Simultaneous Estimation of Carvedilol and Lisinopril In Synthetic Mixture. On the Base of results, obtained from analysis of LIS and CAR is their synthetic mixture and bulk using First Order Derivative Spectroscopy method, it can be concluded that the method has linearity in the range of 10-30μg/ml for LIS and 20- 60 μg/ml CAR. The zero-crossing point (ZCP) was obtained at 228.4 nm and 246.2 nm for estimation of CAR and LIS respectively. The regression coefficient (R2) was found to be 0.9992 and 0.9988 for LIS and CAR at 228.4 nm and 246.2 nm respectively. Limit of detection for LIS and CAR was found to be 0.59 μg/ml and 1.89 μg/ml and Limit of quantification for LIS and CAR was found to be 1.79 μg/ml and 5.73 μg/ml respectively. The % Assay was found to be 99.75 % and 100.53 % for LIS and CAR respectively. Further %R.S.D. was found to be less than 2% for precision, repeatability intraday and Interday study. The % recovery for the LIS and CAR were found to be 99.08- 100.44% and 100.27-100.83% respectively. The methods were validated as per ICH guidelines.

Keywords: Carvedilol, Lisinopril, Synthetic Mixture, First Order Derivative Spectroscopy Method, Development and Validation.