Abstract

TOXICITY STUDY OF FUNGAL CONTAMINATED BREWER’S SPENT GRAIN IN RATS

Jagadeesh S. C.*, Shridhar N. B., Sunilchandra U., Ravikumar C., Manjunatha S. S., Pradeep B. S., Niranjan D., Vinuta M. H., Mohan B. R. and Manjunath Uppar

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Abstract

The present study was undertaken to evaluate the toxicity of fungal contaminated brewer’s spent grain in rats. Repeated dose 28-day oral toxicity study of mycotoxins present in mixed fungal culture filtrates of Rhizopus oryzae 25%, Aspergillus flavus 50% and Aspergillus nomius 25%, was conducted by gavaging it to rats. 40 Wister albino rats were divided in to four groups, 10 rats in each group of either sex. Keeping one group as control, other three groups received the mixed fungal culture filtrates at low dose (0.5 ml/100 g), medium dose (1 ml/100 g) and high dose (2 ml/100 g) rates depending on their body weights respectively. Body weight parameter and blood samples were taken on day 0, 14 and 28 days of the study. Biochemical parameters like ALT, AST, BUN, creatinine and haematological parameters like TLC, TEC, Hb, PCV and Blood clotting time were analysed. Significant (P

Keywords: Brewer’s spent grain, Toxicity, Rat, Rhizopus oryzae, Aspergillus flavus, Aspergillus nomius.