Abstract

ADVANCEMENTS IN LIQUID CHROMATOGRAPHY-MASS SPECTROMETRY: METHOD DEVELOPMENT AND APPLICATIONS

Prakruti Desai*, Grishma Desai, Dr. Ashok Akbari and Dr. Vineet C. Jain

.

Abstract

Liquid chromatography is an advanced technique used to separate multiple analytes with high precision. Mass spectrometry is known for its precise detection capabilities. In the analysis, the coupling effect of LC-MS has proven to be a highly useful and significant analytical tool. LC-MS is widely used in environmental, biomedical, food and pharmaceutical analysis. It has demonstrated applicability to biochemical screening of inherited diseases, therapeutic drug monitoring, and toxicology. LC-MS can accurately detect steroid hormones, vitamins and their related metabolites from biological materials. The creation of novel LC-MS techniques will serve as a tool to help further analyses. LC-MS method development begins with goal setting followed by research and planning. Development of a validated method requires thermodynamic and kinetic considerations as well as chromatographic development with MS optimization and tuning. The use of silica-based core-shell particles, microcolumns, monolithic columns, and hybrid columns is constructive for the future of LC-MS technology.

Keywords: Liquid Chromatography, Mass Spectrometry, Method Development, Biomedical Analysis, Environmental Analysis.