Abstract

OXIDO-REDUCTIVE REACTIONS CATALYZED BY ENZYMES

Michele Vitolo*

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Abstract

This study examines the influence of pH and temperature on the activity of three enzymes: glucose oxidase (GO), glucose 6-phosphate dehydrogenase (G6PDH), and glutamate dehydrogenase (GLuDH). The highest levels of activity for GO, GLuDH, and G6PDH were observed at 30°C, with pH values of 5.0, 7.0, and 7.5, respectively. When these enzymes were dissolved in buffers at their optimal pH levels, they remained stable at 30°C for a duration of 30 hours. Additionally, a viable NADP/NAPH interconversion process was sustained for 30 hours by employing a combination of GLuDH and G6PDH within a membrane reactor, which was intermittently supplied (at a rate of 10 mL/h) with glucose 6-phosphate (G6P) and 2- oxoglutarate/ammonium sulfate, both of which are common substrates for G6PDH and GLuDH, respectively.

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