Abstract

A REVIEW ON METHOD DEVELOPMENT AND VALIDATION OF DOLUTEGRAVIR BY DIFFERENT ANALYTICAL TECHNIQUES

Santhosh Illendula*, Rafiya Jabeen, Pilli Sony, Irfan Alam, Dr. Ch. V. Suresh and Dr. K. N. Venkateswara Rao

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Abstract

Pharmaceutical analysis is a branch of practical chemistry that involves a series of process for identification, determination, quantification and purification of a substance, separation of the components of a solution or mixture, or determination of structure of chemical compounds. The substance may be a single compound or a mixture of compounds and it may be in any of the dosage form. The substance used as pharmaceuticals are animals, plants, micro organisms, minerals and various synthetic products. As a consequence, analytical method development has become the basic activity of analysis. Recent development in analytical methods has been resulted from the advancement of analytical instruments. The improvement of the analytical method development and analytical instruments like UV, HPLC, LC-MS are required for reduced the time of analysis, increased precision and accuracy and reduced costs of analysis. Dolutegravir (DTG) is an antiretroviral medication used, together with other medication, to treat HIV/AIDS. It may also be used, as part of post exposure prophylaxis, to prevent HIV infection following potential exposure. Dolutegravir is an HIV-1 integrase inhibitor that blocks the strand transfer step of the integration of the viral genome into the host cell (INSTI). The effect of this drug has no homology in human host cells, which gives it excellent tolerability and minimal toxicity.

Keywords: Analytical method development, validation, Dolutegravir, UV, HPLC, LCMS.