Abstract

PREVALENCE OF PVL GENE AMONG METHICILLIN RESISTANCE S. AUREUS ISOLATES IN BAGHDAD CITY

Sawsan Mohammed Kareem*, Sawsan Sajid Al- Jubori, Munim Radwan Ali

Abstract

In the current study eighty-four staphylococcal isolates were obtained from patients who were admitted to Baghdad in hospitals during the period July to November 2013 to aim investigation about prevalence of methicillin resistant S. aureus (MRSA) phenotypically and genotypically, on the other hands to determine prevalence of pvl gene among S. aureus isolates additional to screening about antibiogram profile. There were identified (74 out of 84) isolates as S. aureus isolates, the phenotypic screening method about MRSA has been revealed the percentage of MRSA isolates were (74.32%) while genotypic screening method has been revealed (84.43%) harboring with mecA gene. Also the results have been revealed (6.55%) of MRSA isolates harboring of pvl gene. Antibiogram profile of isolates have been revealed completely resistance of MRSA isolates toward cloxacillin, oxacillin and cefepime while some isolates showed high resistance toward Amoxicillin /Clavulinic acid (98.36%) and Ceftazidime (95.2%). the moderate resistance toward tetracycline (31.14%), the low resistance toward azithromycin (24.6%), vancomycin (16.4%), gentamycin (13.11%), ciprofloxacin (13.11%) and finally the lowest resistance toward imipenem (6.55%) .

Keywords: MRSA, femA gene, mecA gene, pvl gene.