Abstract

EVALUATION OF ANTI-OXIDANT AND ANTI-CANCER ACTIVITY OF ROOT EXTRACTS OF ARTOCARPUS HIRSUTUS BY IN-VITRO METHODS

K. V. Vinothini and S. Shervinjose*

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Abstract

Artocarpus hirsutus is native to Kerala and Kanyakumari in India. It belongs to the family Moraceae. In conventional medicine, this species is found to be beneficial in treating acne, diarrhoea and ulcers. Here, we have selected this plant to evaluate antioxidant and anticancer activity. Anti-oxidant activity of Chloroform and ethanolic extracts of A. hirsutus root were studied by DPPH and ABTS method. The chloroform extract showed an IC50 value of 10, 3 μg/ml, and the IC50 value of standard ascorbic acid was 40 μg/ml. The ethanolic extract showed the IC50 value of 7, 1 μg/ml. Using extracts of ethanol and chloroform, the MTT assay was used to evaluate cytotoxic activity in the colon cancer cell line HT-29 and compared to the conventional drug 5-fluorouracil. The IC50 value of chloroform and ethanolic extract was found to be 58.71 μg/ml and 51.05 μg/ml respectively and the IC50 value of 5-fluorouracil is 8.8μg/ml. The ethanolic extract exhibited the greatest inhibition of the two extracts. Therefore, the ethanolic extract was tested for its ability to inhibit the normal Vero cell line and it was found to be less harmful. Further apoptotic investigation of the ethanolic extract was performed using DNA fragmentation and microscopic examination. After being treated with ethanolic extract, the cells revealed a reddish or orange colour and a loss of membrane integrity. This resulted in confirming that Artocarpus hirsutus ethanolic extract had an apoptotic effect on the colon cancerous cell line HT-29. The GC-MS analysis of the root extract of Artocarpus hirsutus identified compounds with drug-like properties based on ADME and toxicity prediction, indicating that this plant is a rich source of phytoconstituents with a high binding affinity in docking experiments. Numerous noteworthy actions of the phytochemicals under investigation were uncovered by the phytoconstituents using the PASS analysis approach.

Keywords: Artocarpus hirsutus, Pharmacology, PASS analysis, Anti-Cancer, Antioxidant, Toxicity prediction, HT-29, ADME properties, VERO cell line, GC-MS, Artocarpus, Moraceae.