Abstract

DEVELOPMENT AND EVALUATION OF ANTIPSORIATIC HERBAL CREAM OF QUERCETIN RICH EXTRACT OF DIOSPYROS EMBRYOPTERIS BARK

Mona Piplani*, Nisha Chaudhary, Saloni Bhatti and Pankaj Bhateja

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Abstract

In the present study, it was envisaged to standardization of plant bark on the basis of presence of quercetin, preparation of herbal cream from quercetin rich extract and its evaluation was carried out. The oil free uncharacterized methanol extract of plant bark, extracted by Soxhlet apparatus showed presence of flavonoids. The optimum resolution of quercetin in plant sample was obtained employing mobile phase containing toluene: ethyl acetate: formic acid in the ratio (7.5:5.5:1). The content of marker compound in plant bark part was found to be 0.875 ± 0.0001% w/w. The parameters mentioned in ICH guidelines were found to be in prescribed limits as described by ICH guidelines. Finally, it can be suggested that quercetin is responsible for various pharmacological actions of plant on the basis of literature records. Further, the dichloromethane extract for preparation of uniform o/w cream was found to provide 15.25 % w/w yield. The pH of herbal cream was noted as 6.6 ± 0.10. No sign of irritation were observed on any skin part with application of herbal cream. The rheometer apparatus was used to check the viscosity of herbal cream and suggested that herbal cream exhibited good pseudo plastic nature. The increment in shear rate suggested the decline in viscosity of herbal cream and further inferring its pseudo plastic nature. At last, the observations of spreadability testing parameters such as firmness and work of shear suggested that prepared herbal cream could be easily applied on infected areas of skin with no signs of separation. The content of herbal plant extract in prepared herbal cream was quantified with the help of UV/VIS spectrophotometry and recorded as 98.85% w/w.

Keywords: Diospyros embryopteris, flavonoid, dichloromethane, herbal cream, psoriasis.