Abstract

STANDARDIZATION AND PHYSIO-CHEMICAL ANALYSIS OF VARMA POLYHERBAL FORMULATION SITRAMUTTI KIYAZHAM- I

Dr. D. Gnana Sekaran*, Dr. N. P. Jeevitha, Dr. I. Karthika and Dr. T. R. Siddique Ali

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Abstract

Introduction: Siddha system of medicine is a traditional system of medicine. Siddha is the mother medicine of ancient Tamils/Dravidians of peninsular South India. The word siddha means established truth. Varmam is the branch of siddha system of medicine. Varma maruthuvam is based on the energy flow that flows throughout the body. Most of the varma medicines are effective but they have not been standardized yet. Standardization of Siddha Varma Polyherbal formulation becomes essential to establish the monograph of the particular formulation along with this, it encompasses the Genuity, purity and safety of the preparations intended for usage in patients. “SITRAMUTTI KIYAZHAM- I (SK-I)”, a polyherbal formulation in varma medicine, is traditionally used to varma kaayam (pain), thodu varmangal, padu varmangal, thatu varmangal. Aim and objective: To standardize “SITRAMUTTI KIYAZHAM- I (SK-I)”, a siddha varma polyherbal formulation using physiochemical analysis and other analytical techniques such as HPTLC and TLC, Heavy metal analysis. Materials and Methods: Varma polyherbal formulation “Sitramutti kiyazham –I (SK-I)”, drug mentioned in the textbook of “Formulary of Varma medicine (Varma marunthu sei muraigal 5th edition)” which is used to treat varma kaayam (pain). A scientific approach was undertaken to standardize the varma polyherbal formulation SK-I by conducting a through physiochemical evaluation, HPTLC and TLC thereby ensuring the establishment of its quality and efficacy parameters. Results: The results obtained from physicochemical evaluation shows that the Total ash value of Sitramutti kiyazham –I (SK-I) was “0.4±0.226%”, in which the acid insoluble ash was “0±0”, loss on drying at 105ºC of the formulation SK-I was noted to be “4.867±0.450” in which water soluble extract value and alcohol soluble extract value was “19.48±2.66”, and “2.81±0.055” respectively. The pH value of the drug 7.15 which is indicates it is slightly alkaline (basic). HPTLC finger printing analysis of the sample reveals the presence of eight prominent peaks corresponds to the presence of eight components present with in it. Heavy metal analysis by AAS result shows no traces of heavy metals such as Mercury, Arsenic, Lead and Cadmium. Microscopic observation of the particle size analysis reveals that the average particle size of SK-I was found to be 112.9±32.5 μm. Conclusion: The preliminary physiochemical analysis of the siddha drug SK-I provides a foundational fingerprint for future clinical studies, there by facilitating the establishment of its safety, efficacy and quality parameters.

Keywords: Sitramutti kiyazham - I, SK-I, Varma polyherbal formulation, Physiochemical analysis, HPTLC.