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World Journal of Pharmaceutical Research

( An ISO 9001:2015 Certified International Journal )

An International Peer Reviewed Journal for Pharmaceutical and Medical Research and Technology

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Abstract

DEVELOPMENT AND VALIDATION OF A RP-HPLC METHOD FOR THE SIMULTANEOUS DETERMINATION OF VITEXIN-2″-ORHAMNOSIDE AND HYPEROSIDE IN LEAVES AND FLOWERS OF HAWTHORN

Andriy Gudzenko*

State Laboratory for Quality Control of Medicines, State Institution �Institute of Pharmacology and Toxicology of National Medical Academy of Science of Ukraine�, Kiyv, Ukraine. 14, Eugene Potie str., 03680, Kyiv, Ukraine.

Abstract

A method for separation and quantification of two flavonoids by reverse-phase high performance liquid chromatography (HPLC) was developed and validated. Flavonoids present in extract of the leaves with flowers of hawthorn (Crataegus pentagyna Waldst. et Kit) were analyzed. Vitexin-2″-O-rhamnoside and hyperoside were used as calibration standards. In this study, an excellent linearity was obtained with r higher than 0.99. Besides, the chromatographic peaks showed good resolution. With other validation data, including precision, specificity, accuracy and robustness, this method demonstrated good reliability and sensitivity, and can be conveniently used for the quantification of vitexin-2″-O-rhamnoside and hyperoside of the extract of the leaves with flowers of hawthorn. In summary, the method above can be considered specific, exact, precise, linear, robust and easy to perform. Further this method can be applied to a standardization of multicomponent herbal remedies, that incorporate leaves with flowers of hawthorn.

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