Abstract

GLYCOCONJUGATE-RECOGNIZING SYSTEMS IN CULTURE FLUIDS OF HUMAN PROBIOTIC STRAINS OF BIFIDOBACTERIA AND LACTOBACILLI

Lakhtin V. M.*, Lakhtin M. V. and Aleshkin V.A.

Abstract

Chemiluminescent and fluorescence analyses were used to obtain and identify visual patterns of protein glycoconjugatebinding/ sensitive/recognizing ordered sets or systems and subsystems (including lectin and glycol-oxidase ones) in culture fluids of probiotic strains of bifidobacteria and lactobacilli isolated from healthy human donors. Glycoconjugate-binding systems recognized glycoconjugates in a mosaic unique way in accordance with the principles: a) "one lectin"-"a limited set of glycoconjugates ranked/ordered by affinity"; b) "one glycoconjugate"-"a limited set of lectins ranked/ordered by affinity"; c) "variable (sub)system of lectins"—"a variable (sub)system of glycoconjugates corresponding to the variation of lectins". Glycoconjugate-binding subsystems of lectins and enzymes of carbohydrate metabolism of probiotic bacteria were characterized by similarities and differences in recognition (the presence of similar and unique components in subsystems), binding (including relative severity, in comparison with other components of the strain subsystem and subsystems of other strains) and distinguished synthetic polymer universal antigenssimilar structures of the broad medical importance and applications.

Keywords: bifidobacteria, lactobacilli, typing, cultural fluid, proteins, lectins, enzymes, glycoconjugates, antigens, probiotics, human.