Abstract

FLUORESCENT AND PEPTIDE CHARACTERISTICS OF THE HUMAN PROBIOTIC BACTERIA CULTURES

Lakhtin V. M.*, Lakhtin M.V. and Aleshkin V.A.

Abstract

A highly sensitive rapid analysis of the intrinsic fluorescence of culture supernatants, their ultrafiltrates and concentrates of proteins and exopolymer substances of Gram positive bacteria on a hydrophobic porous membrane is proposed using the example of probiotic strains of lactobacilli and bifidobacteria and lactobacillar multiprobiotic Acylact. The method was confirmed by analysis using a fluorescent dye SYPRO protein blot stain. In addition, an approach to the determination of partially hydrolyzed protein in fractions of cultures of Gram positive bacteria is described. A method of highly sensitive comparative (ranking of a parameter in sequences) microanalysis of partially hydrolyzed protein in culture supernatants is proposed using the example of lactobacilli probiotic strains and their consortium Acylact. Acylact formulas have been calculated, taking into account the contribution of ingredient strains, in the form of coupled ordered sequences of strains and Acylact - all ranked by the content of cultural peptides and proteins.

Keywords: lactobacilli, bifidobacteria, multistrain consortium, Acylact, cultures, peptides, proteins, ranking, fluorophores, solid-phase assay, live image.