Abstract

DEVELOPMENT OF PROBIOTIC HERBAL ORAL CARE FORMULATION

*Tisha Puthran, Vivek Singh, Dr. Gauri Vahalkar and Dr. Subhashini Mahrana

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Abstract

This formulation is an herbal probiotic powdered mouthwash designed to enhance oral health through natural ingredients and probiotics. Addressing the strong link between poor oral hygiene and systemic health issues such as diabetes and cardiovascular diseases, which provides a safe, eco-friendly alternative to conventional mouthwashes. Formulated with Lactobacillus and Bifidobacterium strains, sage, mint, clove, coconut oil powder, and xylitol, it targets plaque reduction, gum health, and microbiome balance. Analytical results revealed an average moisture content of 5.85%, ensuring probiotic viability below the 6% threshold. The ash content measured 28.37%, reflecting notable mineral content, while the pH of 6 and 0.1% total sugars supports a mild, balanced oral environment. Phytochemical screening confirmed the presence of phenolics, gums & mucilage, and terpenoids contributing to antioxidant and anti-inflammatory benefits. Microbial analysis demonstrated viable probiotic counts (~11.68 × 10^8 CFU/g) and strong antibacterial activity, indicated by a 15mm zone of inhibition against oral pathogens, outperforming select commercial samples. Sensory evaluation yielded a favorable overall acceptance score of 7.5, reflecting high consumer appeal. These findings underscore formulation efficacy in promoting oral health and suggest its potential to fill the market gap for chemical-free, multifunctional oral care products.

Keywords: Herbal mouthwash, probiotics, oral health, sage powder.