Abstract

FORMULATION AND DEVELOPMENT OF HERBAL ETHOSOMAL GEL FOR ANTI-AGING PROPERTY USING ROSEMARY (SALVIA ROSMARINUS) AND KIWI (ACTINIDIA DELICIOSA) EXTRACT

Pankaj Singh Jadaun*, Janki Prasad Rai and Dr. Akhlesh Kumar Singhai

Abstract

This study focuses on the development, characterization, and optimization of an ethosomal formulation designed for anti-aging applications. The formulation consists of Rosemary and kiwi extracts, soy lecithin, and propylene glycol, incorporated into ethosomes and further integrated into Carbopol 934K gel for enhanced topical delivery. Five ethosomal formulations were prepared with varying ingredient concentrations to identify the optimal formulation. The evaluation involved determining entrapment efficiency, particle size, zeta potential, and polydispersity index (PDI). Transmission electron microscopy (TEM) and atomic force microscopy (AFM) were used to analyze surface morphology, while viscosity, pH, spreadability, and extrudability were assessed to confirm the gel’s suitability for topical application. Stability studies examined ethosomal formulations under different conditions over a two-month period. Among the formulations, EF1 emerged as the most optimized, showing an entrapment efficiency of 89.48 ± 0.43%, PDI of 0.14 ± 0.05, zeta potential of 47.7 ± 0.5 mV, and vesicle size of 139.7 ± 1.55 nm. The ethosomal suspension and lyophilized ethosomal suspension demonstrated the best stability at 4°C/60 ± 5% RH. This study concludes that the ethosomal formulation of Rosemary and kiwi extracts provides a synergistic anti-aging effect. The ethosomal system enhances penetration, prolongs drug retention, and ensures targeted and sustained release. With optimized ingredients and processing conditions, the study demonstrates that ethosomal gel formulations are a promising strategy for advanced anti-aging topical treatments.

Keywords: Ethosomes, Rosemary extract kiwi extract, Anti-Aging, Dermal, Transdermal delivery.