Abstract

EVALUATION OF INVITRO AND INVIVO ANTI DIABETIC ACTIVITY AND TOXICITY STUDIES OF PORTULACA QUADRIFIDA L.

Vinjavarapu L. Anusha*, Dr. B. Thangabalan, D. Lakshmi, Ch. Keseena Kishore, G. Lavanya, Y. Navya Reddy, B. Pratap, G.V.N.D. Jahnavi, K. Avinash Goud, J. Rupesh and B. Madhu

.

Abstract

Diabetes mellitus is a major metabolic disorder characterized by chronic hyperglycemia due to insulin deficiency or resistance. Medicinal plants are widely explored for their potential antidiabetic properties. This study investigates the invitro and in vivo antidiabetic activity of portulaca quadrifida along with toxic profile. The invitro study assessed the extract‟s ability to inhibit carbohydrate-digesting enzymes, α-amylase and α-glucosidase, as well as its antioxidant potential. The in vivo study was conducted using alloxan or streptozotocin-induced diabetic animal models, where the extract‟s effect on blood glucose levels, lipid profiles, and pancreatic histopathology was evaluated. Results demonstrated that portulaca quadrifida exhibited significant invitro enzyme inhibition and antioxidant activity. In diabetic animals, oral administration of the extract significantly reduced blood glucose levels, improved lipid metabolism, and showed protective effects on pancreatic tissues. Additionally, acute and sub- chronic toxicity studies revealed no significant physiological, biochemical, or histopathological abnormalities, confirming the plant extract‟s safety at therapeutic doses. Overall, the study suggests that portulaca quadrifida possesses promising antidiabetic activity and a good safety profile, making it a potential natural therapeutic agent for diabetes management. However, further clinical studies are necessary to validate its efficacy and safety in human subjects.

Keywords: .