Abstract

REGENERATION OF PLANTS FROM HYPOCOTYLE AND ROOT EXPLANT OF ENDEMIC AND ENDANGERED TREE TERMINALIA PLALLIDA BRANDIS OF TIRUMALA HILLS.

R. Vinolya Kumari*

Abstract

Callus was induced from Root, Hypocotyle and Leaf explants of Terminalia Pallid on MS medium supplemented with 2, 4-D alone or in combination with the BAP or KN. Adventitious shoot buds were regenerated from hypocotyle and root explants from the callus tissue on the medium containing BAP or KN in combination with NAA. Generally hypocotyls explants were more responsive than root explants and developed shoots on MS + 1mg/l BAP+ 0.5 mg/l NAA. Whereas root explants develop shoots on MS + 1mg/l BAP + 0.05 mg/l NAA the shoot buds grew into shoot on MS + 2mg/l BAP + 0.5mg/l. Rooting was inducing when shoots were transferee to MS + 2mg/l BAP. The regenerated plants were transfer to the soil.

Keywords: KN – Kinetin, BAP – N6 – Benzyl Aminopurine ; IAA – Indole 3 – acetic acid; IBA – Indole butyric acid; NAA – 1 - Napthalene acetic acid.