Abstract

GC-MS DETERMINATION OF BIOACTIVE COMPOUNDS IN AZIMA TETRACANTHA LEAVES

S. Vasthi Gnana Rani and K. Murugaiah*

Abstract

The aim of this study was to carry out for identification of bioactive compounds from methanolic extract of Azima tetracantha leaves by Gas chromatography and Mass spectroscopy (GC-MS). GCMS analysis of methanolic extract was done by standard protocol using the equipment Perkin-Elmer Gas Chromatography–Mass Spectrometry, while the mass spectra of the compounds found in the extract was matched with the National Institute of Standards and Technology (NIST) library. The GC-MS analysis revealed the presence of various compounds like Tetradecanoic acid $$ myristic acid, Pentadecanoic acid ,9,12-Octadecadienoic acid (Z,Z)- $$ cis-,1-(+)-Ascorbic acid 2,6- dihexadecanoate, Heptadecanoic acid, Oleic acid $$ 9-octadecenoic acid (Z), Octadecanoic acid, methyl, Cis--Eicosadienoic acid and Hexadecanoic acid in the methanolic extract of Azima tetracantha. Hence, the Azima tetracantha may have chemopreventive, anticancer, anti-microbial activity, antioxidant and antiinflammatory activity due to the presence of secondary metabolites in the methanolic extract. These findings support the traditional use of Azima tetracantha in various disorders.

Keywords: Gas chromatography and Mass spectroscopy, Azima tetracantha, Phytochemistry.