Abstract

SPECTROPHOTOMETRIC DETERMINATION OF METHYLPHENIDATE IN A PHARMACEUTICAL PREPARATION BY POTASSIUM PERMANGANATE AND SULPHANILIC ACID

Vaishali P. Parastekar and Dr. Vasant Barhate*

Abstract

Two simple and sensitive Spectrophotometric methods are developed for detection of Methylphenidate Hydrochloride. The first method A is based on oxidation of the drug with alkaline potassium permanganate at room temperature (25±°c). The decrease in absorbance of colored Manganate ions was measured at 610 nm. The second Method B is based on the formation of orange color as a result of reaction between drug and diazotised Sulfanilic acid. The absorbance was measured at 510 nm. All parameters affecting the development of the color were investigated and the conditions were optimized. Under the optimum condition, Beer’s law was obeyed in the concentration range 2-18 μg/ml and 6-18 μg/ml for method A and B respectively. Molar absorptivity was found to be 7.014×103 L mol-1cm-1 and 5.395×103 L mol-1 cm-1 respectively. The proposed methods are well suited for determination of Methylphenidate in pharmaceutical formulations.

Keywords: Methylphenidate Hydrochloride, Spectrophotometry, Potassium Permanganate, Daizotised Sulphanilic Acid, Oxidation.