Abstract

STUDY OF EFFICIENCY FOR LACTOBACILLUS FERMUNTUM ON CRYPTOSPORIDIUM PARVUM

Shaima D.Salman and *Sabaa T. Mohammed

Abstract

This study include collected 60 feces sample from calves suffering from severe diarrhea , Cryptosporidium parvum oocyst isolation and purification from feces samples, then the oocysts were used to induce experimental infection in the Immuno supperessed mice. The mice divided into two groups: treatment group and preventive group, the treatment groups inoculated with suspension of bacteria L.fermentum (1×108 cell / 0.1ml), supernatant (0.1ml) and (0.01 ml) of spiramycin drug, the preventive groups had inoculated with suspension of servant bacteria for a period of 10 days before induced infection. The results showed that the preventive groups had a highest efficiency treatment reached (85.91%) and stopped shedding oocysts on the (7th) day after the treatment, while groups treated with suspension of L.fermentum had efficiency treatment was (81.44 %) and stopped shedding oocysts of parasite on the (7th) day but in groups treated with supernatant of bacteria had efficiency treatment (76.33%) and stopped oocysts shedding on the 10th day, treatment groups with spiramycin drug stopped shedding of parasite oocysts till 11th day after treatment and recorded less efficiency treatment reaching (65.14%). Histopathological study, showed that small intestine tissue in the preventive group return to normal forms. The treatment groups with bacteria and it supernatants had simple changes included hydropic degeneration and increase in the number of goblet cells when compared to a group spiramycin which suffered from damage in the mucous layer and the shorten in lengths of the villi with necrosis.

Keywords: Cryptosporidium parvum, L.fermentum, probiotic.