Abstract

IN-SILICO AND IN-VITRO EVALUATION OF ANTIDIABETIC POTENTIAL OF BLEPHARIS MADERASPATENSIS ETHANOLIC EXTRACT

Suresh V.*, Senthilkumar S. K., Jayaseelan K., Johnson Mary A., Punitha Sri R.

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Abstract

Blepharis maderaspatensis, a traditionally used medicinal plant, was investigated for its potential antidiabetic activity using both in-silico and in-vitro approaches. The ethanolic extract of the plant was subjected to phytochemical screening, which revealed the presence of flavonoids, alkaloids, tannins, and phenolic compounds. In-vitro antidiabetic activity was evaluated using alpha-amylase and alphaglucosidase inhibition assays, showing significant dose-dependent enzyme inhibition, comparable to standard antidiabetic drugs. In the in-silico analysis, major bioactive compounds identified via GC-MS were docked against key diabetes-related targets such as alphaglucosidase, alpha-amylase, and PPAR-γ, using molecular docking techniques. The results showed good binding affinities, supporting the potential of these compounds in managing hyperglycemia. The combined findings suggest that the ethanolic extract of Blepharis maderaspatensis possesses promising antidiabetic properties, warranting further pharmacological and clinical investigations.

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