Abstract

EVALUATION OF MICRORNA-21 IN INVASIVE DUCTAL CARCINOMA IN COMPARISON TO CLINICOPATHOLOGICAL STATUS BY STEM-LOOP RT-PCR PROCEDURE.

Shoroq Mohammed Al-Temimi*, Ali Hausin Al-Khafaj, Salim Rashid Al-Aubaidy.

Abstract

Background:- MiR-21 was one of the first oncogenic miRs and as an anti-apoptotic factor, to be characterized, being up-regulated in numerous tumors including BC . A novel miR quantification method has been established using stem–loop RT followed by TaqMan PCR analysis in tissues or culture cells. Aim of study:-Estimation of miR- 21 expression level in fresh tissues of BC / NATs by using stem-loop follow by TagMan real time PCR (RT-PCR) technique and correlate miR-21 gene expression with age of patients and protein expression of ER,PR and HER-2 . Material and methods:- Stem-loop RT-PCR was performed to identify the expression level of miR-21 in 50 IDC samples and their NATs. IHC for ER,PR and HER-2 . Results:- Mean cancer tissue fold change of miR-21 was significantly higher than that of NATs. Majority of cases showed up regulation of miR-21, 96%. No statistical significant associated with age of patients ,protein expression of ER,PR and HER-2 and fold change of miR-21 expression . Conclusion:- Up-regulation of miR-21 expression has been no statistical significant associated with age of patients and protein expression of ER,PR and HER-2.

Keywords: breast cancer,miR-21gene expression ,stem-loop RT-PCR.