Abstract

MONITORING THE EFFICIENCY OF 5- FLUOROURACIL RELEASED FROM SYNTHESIZED COMPOSITE OF POLY ?- AMINO ESTER AS ANTITUMOR AND ANTIBACTERIAL

F.M. Helaly, R.M. Mourad, H.F. Abdel Hamid, S. I. Abd El-Moez, A.M. Soliman*

Abstract

Controlled release of poly β- amino ester loaded with 5- fluorouracil as drug was synthesized through Michael addition polymerization under specific conditions. The 5- fluorouracil was loaded on the prepared polymeric material near the end of reaction. The investigated composite was subjected to release 5- fluorouracil (active agent) in distilled water at room temperature for different time periods. The sustained release of the active agent was measured spectrophotometrically. The cytotoxicity of released 5- fluorouracil was monitoring as an anti-proliferative agent against human liver cancer cell line (HEPG2) as well as antimicrobial against both Gram positive and Gram negative bacteria. The sustained release of the active agent results showed that, poly β- amino ester polymer will be a promising carrier for the antitumour drugs. Results of the in vitro cytotoxic activity of the released 5-fluorouracil showed a sustained antiprolefrative activity against the tested human liver cancer cell line for 26 days. Moreover, results of the antimicrobial study revealed that the most potent inhibitory activity was against Enterotoxigenic S. aureus ATCC 13565 which show hindrance zone of about 48 mm against the tested ref strain.

Keywords: 5-fluorouracil, poly ?- amino ester, drug delivery, invitro- release, anticancer activity, antimicrobial activity.