Abstract

DEVELOPMENT OF HPTLC-UV METHOD FOR COMPARATIVE PHYTOCHEMICAL STUDY OF STEM BARK VERSUS SMALL BRANCHES OF STREBLUS ASPER LOUR

S. C. Verma*, S. Subhani, E. Vashishth, R. Singh, P. Pant, A.K. Mangal, M. M. Padhi, K. S. Dhiman

Abstract

Streblus asper Lour belongs to the family Moraceae. It is a small tree found in tropical countries, such as India, Sri Lanka, Malaysia, the Philippines and Thailand. S. asper is a medicinal plant from Thailand used in folk medicine for the treatment of several inflammatory diseases, treat wounds, skin diseases, filariasis, leprosy, toothache, fever, diarrhea, dysentery. The chromatographic fingerprints of stem bark and small branches of F. racemosa were developed and validated for comparison of phytochemicals. Our results revealed that the chromatographic fingerprints combined with similarity measurement could efficiently identify and distinguish S. asper stem bark and small branches. The phytochemical fingerprint profiling of stem bark and small branches of S. asper were found similar as an official part of S. asper plant i.e. stem bark, therefore small branches may be used in place of stem bark and vice-versa after comparison and confirmation of same pharmacological activities. The method can also be used for identification of different S. asper species and adulterants.

Keywords: Streblus asper, HPTLC–UV detection, phytochemical fingerprint profiling analysis.