Abstract

DEVELOPMENT AND VALIDATION OF UV SPECTROSCOPIC METHOD FOR QUANTITATION OF VALSARTAN TABLET DOSAGE FORM

Gogula Bhargava*, Anusha K., G. Kulayappa*

.

Abstract

The UV spectroscopic method used to measure Tadalafil involves dissolving the drug in dichloromethane and then diluting it with methanol. The absorbance of Tadalafil is measured at a wavelength of 284 nm, with methanol serving as the reference. This method adheres to Beer‘s law within the concentration range of 10 to 40 mcg/ml. The resulting linear equation is y = 0.01013x + 0.10100, and the correlation coefficient is 0.99940. The UV spectroscopic method for measuring Valsartan relies on determining the ultraviolet absorbance maximum of the drug at 230 nm, using acetonitrile as the diluent. The method follows Beer‘s law in the concentration range of 2.5 to 25 mcg/ml, with the linear equation y = 0.05702x - 0.02740 and a correlation coefficient of 0.997. Additionally, the drug obeys Beer‘s law within the concentration range of 2.5 to 15 mcg/ml. Both methods are simple, fast, and do not involve complicated sample preparation steps, making them economical and suitable for routine quality control applications. In conclusion, the developed methods are effective for the analysis of the selected drugs in both bulk and tablet forms.

Keywords: Tadalafil, UV-Spectroscopic method, Valsartan, Absorbance, Beer ‗law, Analytical- techniques, Quality Control, Analyte, Separation Techniques, Immovable phase.