Abstract

AMYLASE ENZYME PRODUCTION USING TAPIOCA WHITE PEELS

S.A.kirubakaran, C.Selvaraj, Haripriya.R, and P.Thirumalai Vasan*

Abstract

Amylases are one of the most important enzymes in present-day biotechnology. The present study was aimed to produce amylase under optimized conditions utilizing White Tapioca peels as substrate. The objectives of the present study includes the analysis of various physical factors that influence the growth and the production of the amylase such as temperature and pH, substrateconcentration,Incubation,effecet of different Carbonsources& Nitrogen sources. Substrate cmc for obtaining maximum yield. B. amyloliquefaciensstrain was obtained from (MTCC1488) and organism exhibited higher amylase activity .On analyzing the physical parameters the optimum incubation time for amylase production is 72 hrs and pH found to be 8.Optimum temperature was found to be 40°C because activity was higher at this temperature. Sucrose and casein were effecetcarbon sources and nitrogen sources respectively. On above mentioned optimum conditions amylase enzyme was produced by B. amyloliquefaciensin higher amount of 2.890 (U/ml).

Keywords: B. amyloliquefaciensstrain,