Abstract

BIOTYPING, SEROTYPING AND PHENOTYPING CHARACTERIZATION OF VIBRIO CHOLERAE O1 ISOLATED IN HYDERABAD

Madhavi.K*  and Gayatri

Abstract

Introduction - Cholera is an acute diarrhoeal disease caused by Vibrio cholerae. Of the 206 serogroups of V.cholerae only O1 and O139 which produce cholera toxin (CTX) are known to cause epidemics. The present study was conducted to determine biotypes, serotypes, phage types of V.cholerae prevalent in Hyderabad and to determine the antibiotic susceptibility pattern of isolates.Materials & Methods :A prospective study was conducted during the period January 2012 to July 2012 at a Tetiary Care Hospital, Hyderabad. During this study period 200 clinically suspected cases of cholera were included. STOOL Specimens were cultured directly on Nutrient agar, Blood agar, MacConkey’s agar, Thiosulfate citrate bilesalt sucrose agar (TCBS), Xylose lysine deoxycholate agar (XLD). Antibiotic suseptibility testing was performed by Kirby Bauer’s Method. Results: Out of 200 samples collected, 62 isolates were of Vibrio cholerae. The predominant age group affected were 15 – 30 years. Males were more susceptible than Females. All the 62 isolates of Vibrio cholerae belonged to Serogroup O1, Biotype ElTor and Serotype Ogawa. All the strains showed 100% resistance to Nalidixic acid, Furazolidine and 99% resistance to Cotrimaxozole. Conclusion:The isolation rate of Vibrio cholerae in our study was 31%. All the 62 Vibrio cholerae isolates belongs to Serogroup O1, Biotype ElTor, and Serotype Ogawa.80% have mild and moderate symptoms and 20% have severe dehydration can leads to death if untreated.T2 was the predominant phage according to Basu and Mukherjee Method.T27 was the predominant phage according to New Typing Scheme. All the isolates showed uniform sensitivity to Amikacin, Gentamycin, Ceftriaxone, Cefotaxime, Doxycycline.All the isolates showed high degree of resistance to Nalidixic acid, Furazolidine, Cotrimoxazole.

Keywords: V.cholerae, Serotype, Biotype, Phage type, CTX.