Abstract

DETERMINATION OF ACTIVE PHYTOCOMPOUNDS FROM FORMULATED SUNSCREEN CREAM CONTAINING PONGAMIA PINNATA LEAVES AND PUNICA GRANATUM PEEL EXTRACT BY HIGH PERFORMANCE THIN LAYER CHROMATOGRAPHY

Sneha Patil*, Bharti Fegade, Urvashi Zamindar and V.H. Bhaskar

Abstract

Objective: The present investigation has been focused on the determination of photo protective activity of the 10% formulated sunscreen cream containing Pongamia pinnata and Punica granatum extract (3:2) in combination. And the quantitative estimation of phytoconstituents from both the extracts and in the formulated Sunscreen cream by High Performance Thin Layer Chromatography (HPTLC) method. Methods: Formulated Sunscreen cream was evaluated for Sunscreen activity. It is reported that Ellagic acid (polyphenol) and Karanjin (flavonoid) is responsible for the Sunscreen activity of Punica granatum and Pongamia pinnata respectively. Therefore Ellagic acid was used as a standard marker for Punica granatum and Karanjin was a Standard marker for Pongamia pinnata. Ellagic acid and karanjin were chromatographed on TLC aluminium sheets silica gel plates using Chloroform: methanol: formic acid (9:1:2 v/v/v) and Toluene: ethyl acetate (7:3 v/v) as Mobile phase respectively. Detection and Quantification of both the extracts as well as formulated sunscreen cream were performed at Lambda 273 nm. Results: The formulated Sunscreen cream has shown good Photoprotective activity. The RF values of standards were 0.23 for Ellagic acid and 0.6 for Karanjin. The total peak areas of the standards and the corresponding peak areas of extracts were composed and the statistical analysis was carried out. Conclusion: From the present findings, it was concluded that there is a presence of Ellagic acid and Karanjin in Punica granatum and Pongamia pinnata respectively. As well as both the standards are present in 10% formulated Sunscreen cream in good quantity indicates Photo-protective effect of the formulation. KEYWORDS:

Keywords: HPTLC, Ellagic acid, Karanjin, Sunscreen activity.