Abstract

DERMATOPHYTE SUSCEPTIBILITIES TO ANTIMYCOTIC DRUGS BY DISC DIFFUSION METHOD

Usha Mishra, Aruna Solanki and P.K. Khatri*

Abstract

Introduction: Superficial mycoses are common fungal infection of keratinized tissue. Now a day’s several option of antifungal agent are available& clinician must now choose among them. Several report of treatment failure & relapse of dermatophytosis are there. So determination of in vitro antimycotic susceptibility is essential. In our study we evaluate antifungal activity of five antimycotic agents. Material & methods: Sixty seven dermatophyte were isolated from patient having dermatophytosis . Five antimycotic discs viz. nystatin, fluconazole ,ketoconazole ,itraconazole, clotrimazole were used by disc diffusion method for in vitro susceptibility testing against these isolates. Result: A total of sixty seven dermatophytes were isolated and identified. The isolates belong to two genera and four species as follows: T. mentagrophytes 16(23.8%),T. rubrum 38(56.7%), T. violaceum 7(10.4%), M. canis 6(7.58.9%). Regarding the data, it was revealed that nystatin and itraconazole were the most effective antimycotic drugs and clotrimazole had the poorest activity. Conclusion: The disc diffusion method is a simple, reliable, inexpensive and easily adaptable assay which is more practical, simple and easier in comparison to micro dilution method.

Keywords: Dermatophyte , Antimycotic drugs, Disc diffusion method.