Abstract

EVALUATION OF ANTITUMORAL ACTIVITY OF WHOLE PLANT EXTRACT OF PHYLLANTHUS ACIDUS IN A549 CELL LINES

*S. Sri Vikram, S. Parthiban, S. Mohan

Abstract

Cancer is a disease in which some of the body‟s cells grow uncontrollably and spread to other parts of the body. The pathogenesis of lung adenocarcinoma is complex, involving multiple mutations of proto-oncogenes and the activation of various intracellular molecular signaling pathways. The aim of the present study is to evaluation of antitumoral activity of whole plant extract of Phyllanthus acidus in A549 cell line. The study employs an integrative approach encompassing whole plant of extraction material with ethanol and Preliminary Phytochemical analysis with Quantification of total Phenol and Flavonoid content and futher Cell line studies were evaluated such as Cell viability assay, Scratch healing assay, Apoptosis assay. Results include that Total phenolic and Total flavonoid content in EPA was found to be 71.37mg/g and be 40.64 mg/g of extract calculated as Gallic acid and Quercetin equivalent respectively. In Cell viability assay, the percentage of cell viability is decreased at 44% at 300 μM concentration. Mostly the reduction happens at identified IC50 value246.5 μM respectively. A549 cells reductions in the viability of cells were identified with high concentration of the extract. In Apoptosis assay, also seen in other concentration also, but high apoptotic index is not noticed in any concentration. A549 cells treated with EPA (25 200 µM) of phyllanthus acidus extract. In Scratch healing assay particularly noticeable in the (50-200 μM) concentration range compared to the control. The anticancer activity of the phyllanthus acidus extract also inhibits migration of A549 cells and it doesn‟t promote invasion. Isolation and characterization of the chemical constituents present in the phyllanthus acidus extract and exploration of molecular targets in cancer cells will be the efficient process to eradicate cancer in future.

Keywords: Cancer, Phyllanthus acidus, Antitumoral, IC50, Cell viability, Scratch healing, Apoptosis.