Abstract

LC-MS METABOLIC PROFILE OF THE HYDRO-ETHANOLIC EXTRACT OF ZANTHOXYLUM ZANTHOXYLOIDES STEM BARK HARVESTED IN KORHOGO (IVORY COAST)]

Ouattara Logopho Hyacinthe, N’guessan Patrick Audrey, Bamba Souleymane, Mouho Guéi Didier Roselin, Philippe Jéhan, Fabian Lambert, Nicolas Le Yondre, Sima Obiang Cédric, Ambeu-Loko N’ta Christelle Mélissa*, Kablan Ahmont Landry Claude 

Abstract

The traditional uses of Zanthoxylum zanthoxyloides in West Africa have sparked increased interest in the chemical characterization of its plant extracts with a view to their pharmacological exploitation. This study presents the metabolic profile obtained by liquid chromatography coupled with mass spectrometry (LC-MS) of the hydro-ethanolic extract (70/30: v/v) of the bark of Zanthoxylum zanthoxyloides stems harvested in Korhogo (Ivory Coast). Extraction, carried out by maceration for 24 hours, yielded an extract rich in polar and semi-polar metabolites. LC-MS analysis performed in positive mode revealed twenty-one (21) compounds, including flavonoids, coumarins, phenylpropanoid amides, lignans, and alkaloids. In negative mode, nine (09) metabolites were identified, mainly glycosides, fatty acids, and phenolic derivatives. The results reveal a diverse metabolic richness and suggest compounds that may exert antioxidant, vasculoprotective, hepatoprotective, analgesic, antiparasitic, anticancer or anti-inflammatory activities. These data provide a reliable basis for future studies on the isolation, absolute quantification and targeted biological evaluation of molecules of interest.

Keywords: Zanthoxylum zanthoxyloides, LC-MS, secondary metabolites, stem bark, hydro-ethanolic extraction, Ivory Coast.